Catalog Number - 59518-15P

MicroBee C18™ is a traditional octadecylsilane (C18) reversed-phase HPLC stationary phase that has been trusted by chromatographers for many years. As part of the Cogent™ Similars product line, it provides a cost-effective alternative for established analytical methods while delivering the hydrophobic retention characteristics expected from classical C18 chemistry. The end-capped, Type-A silica support is bonded with a straight-chain C18 ligand, making it particularly useful for laboratories operating legacy methods and compendial procedures.

59518-15P is a 150 mm x 4.6 mm ID analytical column packed with 5 µm, 125Å MicroBee C18 particles. The 150 mm length provides an excellent balance of resolution and analysis time, making it well suited for routine analytical testing, quality control, and method development applications. The 5 µm particle size delivers greater efficiency and sharper peak shapes than 10 µm packings, while the industry-standard 4.6 mm ID format typically operates optimally at flow rates of approximately 1.0–2.0 mL/min on conventional HPLC systems.

This USP L1 column is an excellent alternative for methods developed on Microbondapak™ and similar C18 columns. The traditional C18 chemistry is ideal for hydrophobic and neutral compounds analyzed under reversed-phase conditions and is particularly effective for long-chain fatty acids, pharmaceutical compounds, and other lipophilic analytes. Its combination of classical selectivity and improved efficiency makes it a practical choice for both legacy method replacement and new method development.

Key Benefits

• Traditional end-capped C18 stationary phase

• Suitable alternative for USP L1 methods

• Compatible with many legacy Microbondapak™ methods

• Higher efficiency with 5 µm particles

• Improved peak shape and chromatographic performance

• Excellent for hydrophobic and neutral compounds

• Effective for long-chain fatty acid analysis

• Cost-effective Cogent Similars column

Typical Applications

• USP L1 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Quality control testing

• Method development

• Long-chain fatty acid analysis

• Lipophilic compound separations

• Routine analytical HPLC

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee C18

• USP Classification: L1

• Particle Size: 5 µm

• Particle Type: Type-A Silica

• Bonded Phase: C18 (Octadecylsilane)

• Endcapped: Yes

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Traditional end-capped C18 chemistry

• 5 µm particles provide higher efficiency and sharper peaks

• Compatible with conventional HPLC systems

• Industry-standard 4.6 mm ID analytical format

• Excellent alternative for many USP L1 methods

• Suitable for hydrophobic and neutral compounds

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